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About the BookAbout the AuthorTable of Contents

Molecular Biotechnology: Principles and Practices is intended as a textbook aimed at providing undergraduate and postgraduate students with a strong base in this emerging and highly promising interdisciplinary science. It strikes a balance between two important aspects of the science – the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is that it covers a wide range of molecular techniques in biotechnology and is designed to be a student- and teacher-friendly textbook. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The book can also serve as reference to the interested reader who is venturing into the field of biotechnology for the first time. Special Features -Provides comprehensive and up-to-date coverage of key concepts in biotechnology - Logical format used to provide easy access to the information - Clear and well-labelled figures - Extensive cross–referencing between chapters

Channarayappa received his B.Sc. (Ag.), M.Sc. (Ag.), and Ph.D. degrees from the University of Agricultural Sciences, Bangalore. He then went on to earn a second Ph.D., in Genetics and Developmental Biology from West Virginia University, USA, in 1990. He worked as a Postdoctoral Fellow and Postdoctoral Research Associate at the University of Texas MD Anderson Cancer Center, Houston. He was the recipient of the Jawaharlal Nehru National Award for Outstanding Post-graduate Agricultural Research (1993) for significant research contribution in genetics and plant breeding, awarded by the Indian Council of Agricultural Research, New Delhi. He has been teaching both undergraduate and postgraduate students of molecular biology and biotechnology courses for more than 20 years. He is the author of many scientific papers resulting from research conducted in plant sciences, mutation research, and cancer biology.

PART I : Introduction to Biotechnology

1. Biotechnology: Scope and Importance

2. Biosafety and Good Laboratory Practices

PART II: Advanced Techniques in Molecular Biology

3. Techniques of Cell Fractionation and Centrifugation

4. Chemical Synthesis of Nucleic Acids

5. DNA Chip Technology and its Potential Applications

6. Bioinformatics in Biotechnology

PART III: Working with Nucleic Acids

7. Isolation of Nucleic Acids

8. Measuring Nucleic Acid Concentration and Purity

9. Electrophoretic Techniques

10. DNA Sequencing

11. Genetic Maps and Marker Analysis

12. Polymerase Chain Reaction (PCR)

13. In Situ Hybridization

PART IV: Recombinant DNA and Genetic Engineering

14. Fundamentals of Recombinant DNA Technology

15. Enzymes in Molecular Cloning

16. Gene Constructs and Cloning Vectors

17. DNA Libraries 18. Molecular Biology of Gene Transfer Systems

19. Selection and Screening of Recombinant Molecules

PART V: Applications of Biotechnology

20. Genetic Engineering of Microorganisms

21. Genetic Engineering of Animals

22. Genetic Engineering in Plants

PART VI: Working with Proteins

23. Protein Purification Techniques

24. Protein Detection and Estimation

25. Protein Fractionation Techniques

26. Immunochemical Techniques

PART VII: Bacterial and Mammalian Cell Culture

27. Biology of Bacteria

28. Cultivation of Mammalian Cells In vitro

PART VIII: In Vitro Plant Cell Culture and Crop Improvement

29. Plant Cell Culture Laboratory and Requirements

30. Plant Culture Media, Preparation, and Culture Initiation

31. Micropropagation

32. Cultures of Organized Tissues

33. Culture of Unorganized Tissues

34. Cryopreservation and Distribution of Clonal Material

35. Measurement of Plant Cell Growth and

PART VIII: Cytological Analysis

36. Protoplast Fusion and Somaclonal Variation

37. Application of Plant Cell, Tissue and Organ Culture

PART IX: Environmental Biotechnology

38. Biotechnology in Pollution Control

39. Biodiversity and Genetic Conservation

40. Bioenergy Fuel from Biomass

41. Regulatory Aspects of Using Genetically-Modified Organisms

42. Intellectual Property Rights and Socio-Legal Aspects of Biotechnology Appendices